Abstract
Using mouse hybridoma monoclonal antibodies to DNA from MRL/lpr and NZB X NZW (B/W F1) mice, the reactivity of anti-DNA antibodies to several phospholipids was analysed. The anti-DNA antibody which reacted with the common antigenic determinants on the phosphate-sugar backbone of nucleic acid could bind to the cardiolipin, but failed to bind to other phospholipids, including VDRL antigen. We tentatively conclude that the anti-cardiolipin antibody is identical with the anti-DNA antibody, but differs from the BFP reactor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Antinuclear / immunology*
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Antibodies, Monoclonal / immunology
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Antibody Specificity*
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Cardiolipins / immunology*
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Cholesterol / immunology
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DNA / immunology*
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DNA, Single-Stranded / immunology
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False Positive Reactions
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Hybridomas / immunology
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Mice
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Phosphatidylcholines / immunology
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Phosphatidylinositols / immunology
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Syphilis Serodiagnosis*
Substances
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Antibodies, Antinuclear
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Antibodies, Monoclonal
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Cardiolipins
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DNA, Single-Stranded
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Phosphatidylcholines
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Phosphatidylinositols
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DNA
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Cholesterol