Tc-99m labeled HIDA imaging has been used to investigate suspected biliary leaks following orthotopic liver transplantation. In two patients the diagnosis of bile leakage was confirmed and appropriate surgical intervention undertaken. In a third patient, despite clinical suspicion, no leakage was apparent on HIDA imaging, and unnecessary surgery was avoided. HIDA imaging is a useful, noninvasive technique for confirming biliary leakage after liver transplantation.