Using immunofluorescent techniques we have examined the distribution of the glycoproteins, fibronectin and laminin, in bovine eyes. As expected, both fibronectin and laminin were present in blood vessel walls and in all basement membranes, including the internal limiting membrane of the retina. Fibronectin, but not laminin, was observed arranged in fine fibrillar arrays within the vitreous body. The presence of fibronectin in the vitreous cortex and the occurrence of both proteins in the internal limiting membrane of the retina provide a morphologic suggestion of a role for fibronectin and laminin in vitreoretinal adhesion.