Abstract
A purification procedure for a protein obtained from an pathogenic strain of E. coli is described. The protein-called CNF-is active in inhibiting the duplication of cultured mammalian cells. Since nuclei division is apparently normal, treatment of cultured cells with CNF leads to the formation of gigantic, polynucleated cells. The purified protein is chromatographically and electrophoretically homogeneous. A partial characterization of CNF protein is also given.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acids / analysis
-
Bacterial Proteins / isolation & purification*
-
Bacterial Proteins / pharmacology
-
Bacterial Toxins / isolation & purification*
-
Bacterial Toxins / pharmacology
-
Biological Assay
-
Cell Division / drug effects
-
Chromatography, Gel
-
Escherichia coli / physiology*
-
HeLa Cells / drug effects
-
HeLa Cells / physiology
-
Humans
-
Molecular Weight
-
Virulence
Substances
-
Amino Acids
-
Bacterial Proteins
-
Bacterial Toxins