Nuclear immunofluorescence by a monoclonal antibody against microtubule-associated protein-1 as it is associated with cell proliferation and transformation

Exp Cell Res. 1984 Nov;155(1):33-42. doi: 10.1016/0014-4827(84)90765-1.

Abstract

Monoclonal antibody against microtubule-associated protein-1 produced intranuclear immunofluorescent spots, which disappeared under growth-inhibited conditions caused by serum starvation and saturated cell density in untransformed cells. A change of medium to 10% serum gave rise to the reappearance of nuclear spots before the resumption of DNA synthesis. This reversible change of immunofluorescence was also caused by a temperature shift in rat 3Y1 cells transformed by Simian virus-40-A640 (temperature-sensitive in large T-antigen). The fluorescence decreased during S phase of the cell cycle. In contrast the transformed cells always showed nuclear fluorescence, irrespective of serum concentrations or the cell cycle. Growth-inhibited cells previously treated with detergent and salt revealed nuclear fluorescent spots. This result suggested antigenic modification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Cell Cycle*
  • Cell Nucleus / metabolism*
  • DNA Replication
  • Detergents / pharmacology
  • Fluorescent Antibody Technique
  • Growth Substances / pharmacology
  • Interphase
  • Microtubule-Associated Proteins / immunology
  • Microtubule-Associated Proteins / metabolism*
  • Rats
  • Sodium Chloride / pharmacology
  • Temperature

Substances

  • Antibodies, Monoclonal
  • Detergents
  • Growth Substances
  • Microtubule-Associated Proteins
  • Sodium Chloride