Abstract
Spontaneously arising class switch variants of the Genox 3.53 hybridoma cell line were isolated. They secrete IgG2a or IgG2b monoclonal antibodies of anti-DC1 specificity identical to that of the IgG1 secreting parental cell. In contrast to the parental monoclonal antibody, those secreted by the variants are cytotoxic to peripheral blood B lymphocytes of DC1 positive individuals and are thus compatible with existing HLA typing techniques. This provides a general method for converting noncytotoxic anti-HLA antibodies into cytotoxic typing reagents.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology*
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Binding Sites, Antibody
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Cell Separation
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Cytotoxicity Tests, Immunologic
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Flow Cytometry
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Genetic Variation
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Goats
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Humans
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Hybridomas / immunology*
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Immunoglobulin Allotypes / genetics
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Immunoglobulin Allotypes / immunology
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Immunoglobulin G / classification
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Immunoglobulin G / genetics
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Immunoglobulin G / immunology*
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Immunoglobulin Heavy Chains / genetics*
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Mice
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Mice, Inbred Strains
Substances
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Antibodies, Monoclonal
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Immunoglobulin Allotypes
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Immunoglobulin G
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Immunoglobulin Heavy Chains