Abstract
The frequency of lambda light chain (L) producing cells in immunoglobulin-producing cells that have been generated in vitro from bone marrow pre-B cells was investigated. The frequency of lambda-producing cells obtained in such a culture was three- to eightfold higher than that observed in the culture of mature spleen B cells. These results suggest that the activation of lambda gene at pre-B cell stage occurs far more frequently than the frequency presumed from the percentage of lambda-bearing cells in mature B cells.
MeSH terms
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Animals
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Antibodies, Anti-Idiotypic / immunology
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Antibody Specificity
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Antibody-Producing Cells / immunology
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B-Lymphocytes / cytology
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B-Lymphocytes / immunology*
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Bone Marrow Cells
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Cell Differentiation
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Hemolytic Plaque Technique
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Immunoglobulin Light Chains / genetics*
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Immunoglobulin kappa-Chains / genetics
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Immunoglobulin lambda-Chains / genetics*
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Immunoglobulin lambda-Chains / immunology
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Lymphocyte Activation*
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Stem Cells / immunology*
Substances
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Antibodies, Anti-Idiotypic
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Immunoglobulin Light Chains
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Immunoglobulin kappa-Chains
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Immunoglobulin lambda-Chains