Effect of low-NaCl medium on the envelope glycoproteins of Sindbis virus

J Virol. 1978 Mar;25(3):764-9. doi: 10.1128/JVI.25.3.764-769.1978.

Abstract

Lowering the NaCl concentration of the medium inhibits the release of Sindbis virus from infected chicks cells at a stage after the nucleocapsids have bound to the membranes of the infected cells. The failure of trypsin treatment to release the inhibited virus and the ratio of the proteins in the inhibited cells make it seem likely that the inhibited virus is all intracellular. Experiments using antisera specific for E1 and E2, the envelope glycoproteins of Sindbis, suggest that the inhibitory effect of low-salt medium is mediated through an effect on E2. Lactoperoxidase radioiodination experiments indicate that, even when cleaved from PE2, E2 is not exposed on the surface of low-NaCl-treated chick cells.

MeSH terms

  • Cell Line
  • Cell Membrane / drug effects
  • Culture Media
  • Glycoproteins / metabolism*
  • Immune Sera / pharmacology
  • Lactoperoxidase
  • Morphogenesis / drug effects
  • Sindbis Virus / drug effects
  • Sindbis Virus / metabolism*
  • Sodium Chloride / pharmacology*
  • Viral Proteins / metabolism*

Substances

  • Culture Media
  • Glycoproteins
  • Immune Sera
  • Viral Proteins
  • Sodium Chloride
  • Lactoperoxidase