Abstract
Expression of the cloned levansucrase gene (sacB) was demonstrated in E. coli minicells by assay of the enzyme in crude extracts, SDS-polyacrylamide gel electrophoresis and immunoblotting. The existence of a precursor form of the enzyme of MW 53000 was also demonstrated and confirmed by the DNA sequence corresponding to the NH2 terminal region of the protein.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacillus subtilis / enzymology*
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Bacillus subtilis / genetics
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Base Sequence
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Cloning, Molecular*
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DNA, Bacterial / genetics
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Enzyme Precursors / genetics
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Escherichia coli / enzymology
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Genes*
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Genes, Bacterial*
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Hexosyltransferases / genetics*
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Plasmids
Substances
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DNA, Bacterial
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Enzyme Precursors
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Hexosyltransferases
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levansucrase