Abstract
The chemiluminescence of fluorescein derivatives is greatly enhanced when they are conjugated to proteins. We exploited this property in devising a chemiluminescence immunoassay for rubella antibodies. In this sequential "sandwich"-type assay, tubes coated with rubella antigen are used for antibody separation and goat antibody to human IgG conjugated with fluorescein isothiocyanate is used for detection of bound rubella antibodies.
MeSH terms
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Antibodies, Viral / analysis*
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Antibodies, Viral / standards
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Calcium
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Calcium Compounds*
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Fluorescein-5-isothiocyanate
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Fluoresceins
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Fluorescent Antibody Technique
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Humans
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Luminescent Measurements
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Radioimmunoassay
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Rubella virus / immunology*
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Sodium Hypochlorite
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Thiocyanates
Substances
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Antibodies, Viral
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Calcium Compounds
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Fluoresceins
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Thiocyanates
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calcium hypochlorite
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Sodium Hypochlorite
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Fluorescein-5-isothiocyanate
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Calcium