Abstract
A gene-cloning approach was used to determine the transcription polarity of the carbamoylphosphate operon (carAB) of Escherichia coli. In agreement with the accompanying paper (J. Bacteriol. 143:914-920, 1980), our results lead to the conclusion that carA is the proximal gene of the carAB operon.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriophage lambda / genetics
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Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing) / genetics*
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Cloning, Molecular / methods
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Escherichia coli / genetics*
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Gene Expression Regulation
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Genes, Regulator
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Ligases / genetics*
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Operon*
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Plasmids
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Transcription, Genetic
Substances
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Ligases
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Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)