Affinity chromatography of phenylalanine hydroxylase. The structure of a pteridine adsorbent

Eur J Biochem. 1978 Apr 17;85(2):357-63. doi: 10.1111/j.1432-1033.1978.tb12247.x.

Abstract

1. Four independent methods have established that the structure of a previously reported pteridine affinity adsorbent, 6,7-dimethyl-5,6,7,8-tetrahydropterin--CH-Sepharose, is 5(CH-Sepharosyl)-6,7-dimethyl-5,6,7,8-tetrahydropterin. 2. A novel reaction, the carbodiimide-promoted coupling of a carboxyl group to N-5 of a tetrahydropterin, is described. 3. Two novel adsorbents, 5-formyl-tetrahydrofolate--AH-Sepharose and 5-methyl-tetrahydrofolate--AH-Sepharose, are described which may be useful not only in the study of phenylalanine hydroxylase but also in the study of folate-metabolizing enzymes.

MeSH terms

  • Adsorption
  • Chromatography, Affinity / methods
  • Phenylalanine Hydroxylase / isolation & purification*
  • Pteridines

Substances

  • Pteridines
  • Phenylalanine Hydroxylase