Isoelectric focusing in polyacrylamide gel carried out with a simple device for power regulation. Application to mammalian growth hormones

J Chromatogr. 1978 Mar 1;145(2):257-64. doi: 10.1016/s0378-4347(00)81346-8.

Abstract

A unit is described to regulate the electrical power delivered to an isoelectric focusing cell. The device consists in a resistance set, in series with a power supply, which can be adjusted to a suitable resistance value in order to permit only minor changes in wattage. Simultanoeus monitoring of voltage and current is also carried out with a pen-recorder adaptor. The improvements in operative conditions achieved by these means are tested by the fractionation of mammalian growth hormones.

MeSH terms

  • Animals
  • Anura
  • Cats
  • Electrophoresis, Polyacrylamide Gel
  • Growth Hormone / analysis*
  • Humans
  • Isoelectric Focusing / instrumentation*
  • Rats
  • Sheep
  • Swine

Substances

  • Growth Hormone