Liquid chromatographic determination of nicarbazin in feeds and premixes

J Assoc Off Anal Chem. 1984 Nov-Dec;67(6):1115-7.

Abstract

A liquid chromatographic (LC) method has been developed for the determination of nicarbazin in premixes and poultry feed. Liquid chromatography of the 4,4'-dinitrocarbanilide (DNC) portion of nicarbazin is performed isocratically with a reverse phase octadecylsilica column and a UV detector set at 340 nm. The 2-hydroxy-4,6-dimethylpyrimidine (HDP) portion of nicarbazin is chromatographed isocratically with a reverse phase octylsilica column and a UV detector set at 305 nm. Nicarbazin concentration can be calculated by assaying both DNC and HDP, or by assaying DNC or HDP and assuming that nicarbazin is a 1:1 molar ratio of the two. Average recoveries of DNC and HDP added to poultry feed were 101% and 87%, respectively. This procedure provides an alternative to existing colorimetric procedures for determining nicarbazin in premixes and poultry feeds.

MeSH terms

  • Animal Feed / analysis*
  • Carbanilides / analysis*
  • Chromatography, Liquid / methods
  • Food Additives / analysis*
  • Nicarbazin / analysis*
  • Pyrimidinones / analysis

Substances

  • Carbanilides
  • Food Additives
  • Pyrimidinones
  • 2-hydroxy-4,6-dimethylpyrimidine
  • Nicarbazin
  • 4,4'-dinitrocarbanilide