Complement-component-C3-opsonized immunoglobulin G anti-DNA antibodies do not bind effectively to red blood cells unless aggregated on a high-Mr DNA matrix

Biochem J. 1984 Dec 15;224(3):755-9. doi: 10.1042/bj2240755.

Abstract

Large, soluble ds (double-stranded) DNA-IgG (immunoglobulin G) anti-dsDNA immune complexes (greater than or equal to 200 S) that were previously opsonized with complement were digested with DNAase. The small complement-component-C3-fragment-labelled IgG (11-14 S) that was then isolated did not bind effectively to complement receptor type 1 on human red blood cells. However, when this IgG was immune-complexed with 3H-labelled PM2 (bacteriophage directed against a marine Pseudomonas) dsDNA (Mr approximately 6 X 10(6), substantial binding of both the DNA antigen and IgG to the erythrocytes was demonstrable.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigen-Antibody Complex / immunology*
  • Centrifugation, Density Gradient
  • Complement C3 / immunology*
  • DNA / immunology*
  • Erythrocytes / immunology*
  • Humans
  • Immunoglobulin G / immunology*
  • In Vitro Techniques
  • Molecular Weight

Substances

  • Antigen-Antibody Complex
  • Complement C3
  • Immunoglobulin G
  • DNA