Modifications of leucine transport system of mouse oocytes have been studied throughout Graafian follicle development and oocyte maturation. In contrast to sheep oocytes (Moor and Smith, 1979), in the mouse kinetic constants and efflux rate of leucine transport system did not vary in diestrus, proestrus, and metaphase II (met II) oocytes. However, kinetics of leucine equilibration in proestrus and met II oocytes was significantly slower than that found in diestrus cells, and this may reflect a decreased availability of internal amino acids for exchange.