Abstract
Cytosolic [Ca2+] has been measured by using the Ca2+-sensitive indicator quin2 in rat liver cells. Optimal loading and hydrolysis have been obtained by equilibrating the cells with 50 microM quin2 acetoxymethyl ester for 150 s. The increase in [Ca2+]i initiated by noradrenaline and vasopressin was reduced but not abolished by removing external Ca2+.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aminoquinolines
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Animals
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Calcium / analysis*
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Fluorescent Dyes
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Liver / analysis*
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Liver / drug effects
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Norepinephrine / pharmacology*
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Propranolol / pharmacology
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Rats
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Vasopressins / pharmacology*
Substances
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Aminoquinolines
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Fluorescent Dyes
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Vasopressins
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Quin2-acetoxymethyl ester
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Propranolol
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Calcium
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Norepinephrine