Abstract
In the classic peptidergic neurosecretory system of vertebrates the neurohormone oxytocin is associated with the carrier protein neurophysin. By using small gold particles (3 to 12 nm) in the protein A-gold technique, both the hormone and the carrier-protein will be labelled, while larger particles (20 nm) label only the neurophysin. To bind the latter particles, a greater number of Fc-regions of antigen-IgG-complexes seems to be necessary.
MeSH terms
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Animals
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Gold
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Hypothalamus, Anterior / analysis*
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Immune Sera
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Immunoglobulin Fc Fragments
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Immunologic Techniques*
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Neurophysins / analysis*
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Oxytocin / analysis*
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Paraventricular Hypothalamic Nucleus / analysis
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Pituitary Gland, Posterior / analysis*
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Rats
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Rats, Inbred Strains
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Staphylococcal Protein A
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Supraoptic Nucleus / analysis
Substances
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Immune Sera
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Immunoglobulin Fc Fragments
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Neurophysins
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Staphylococcal Protein A
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Oxytocin
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Gold