Structure and location of asparagine-linked oligosaccharides in the Fc region of A human immunoglobulin D

Biochem Biophys Res Commun. 1983 Jan 14;110(1):181-6. doi: 10.1016/0006-291x(83)91277-9.

Abstract

Seven kinds of asparagine-linked oligosaccharides were bound to the Fc region of a human immunoglobulin D(NIG-65). The oligosaccharides quantitatively released from four species of glycopeptides by digestion with almond glycopeptidase, were separated by Bio-Gel p-4 column chromatography and were purified further by thin-layer chromatography. The sugars were identified with GC-MS following the permethylation of respective oligosaccharide. To Asn-68 (NIG-65 Fc numbering (1)), two kinds of high-mannose-type oligosaccharides were bonded. To Asn-159, a kind of hybride-type and two kinds of bisected complex-type oligosaccharides were attached. From Asn-210, four kinds of bisected complex-type oligosaccharides were isolated.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Asparagine / analysis*
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Chickens
  • Female
  • Fibrinogen
  • Glycopeptides / isolation & purification*
  • Humans
  • Immunoglobulin D*
  • Immunoglobulin Fc Fragments*
  • Oligosaccharides / analysis*
  • Ovalbumin
  • Quail
  • Structure-Activity Relationship

Substances

  • Glycopeptides
  • Immunoglobulin D
  • Immunoglobulin Fc Fragments
  • Oligosaccharides
  • Asparagine
  • Fibrinogen
  • Ovalbumin