Edwardsiella tarda was isolated from patients, water tortoises (Clemmys caspica), and samples of water from Lake Kinnereth, the river Jordan, well water, and sewage water. Of the 53 isolates, 35 belonged to completely identified serotypes, among them 7 new ones. Fourteen cultures had O antigens, and one had an H antigen, different from those previously described. Three serotypes isolated from patients were also found in other sources: water tortoises, lake water, or both.