Thirty kidneys from nine embryos, 20 fetuses, and one full-term baby were examined for their renin content by immunofluorescence and the peroxidase antiperoxidase method, using an antihuman renin antiserum. Renin-containing cells were found in the early metanephros (5-week-old fetuses). Most of them were located in the wall of well-developed renal arteries in the vicinity of the prospective vascular pole of the glomeruli. In the poorly differentiated peripheral renal cortex, intracellular fluorescence was seen in nearby arterioles of pocket-like s-shaped tubules. Rarely, labeled cells were found in the wall of major branches of renal arteries. In all locations, the renin-containing cells appear to be clearly linked to the development of the renal vascular system.