Protein synthesis using poly(2'-halogeno-2'-deoxyadenylic acids) as messenger

Biochim Biophys Acta. 1982 May 31;697(2):174-7. doi: 10.1016/0167-4781(82)90074-4.

Abstract

Poly(2'-fluoro-2'-deoxyadenylic acid), poly(2'-chloro-2'-deoxyadenylic acid) and poly(2'-bromo-2'-deoxyadenylic acid) are used as messenger RNAs in protein synthesizing systems in vitro. All polynucleotides were active as messengers and [14 C]lysine was incorporated into polypeptides. The initial velocity of polylysine formation was greater using poly(2'-fluoro-2'-deoxyadenylic acid) as messenger than in the case of poly(rA), and all synthetic messengers lived longer in the protein synthetic system.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Escherichia coli / genetics
  • Kinetics
  • Poly A* / genetics
  • Polydeoxyribonucleotides*
  • Protein Biosynthesis*
  • RNA, Messenger / genetics*
  • RNA, Transfer / genetics
  • Structure-Activity Relationship

Substances

  • Polydeoxyribonucleotides
  • RNA, Messenger
  • Poly A
  • poly(2'-chloro-2'-deoxyadenylic acid)
  • poly(2'-bromo-2'-deoxyadenylic acid)
  • poly(2'-fluoro-2'-deoxyadenylic acid)
  • RNA, Transfer