[Protein globule without the unique three-dimensional structure: experimental data for alpha-lactalbumins and general model]

Biofizika. 1982 Nov-Dec;27(6):1005-16.
[Article in Russian]

Abstract

It has been shown that bovine and human alpha-lactalbumins under the influence of acidic pH, high temperatures or low guanidine hydrochloride concentrations are transferred into a state which is quite distinct both from the native and the completely denatured (unfolded) ones. Human alpha-lactalbumin can be transferred into this intermediate state also by removal of the bound to it Ca2+ ion. On the basis of the obtained physical characteristics of this state a model of the "intermediate" state is suggested in which a protein globule is compact, has a secondary structure, but does not possess a unique three-dimensional structure. Phase diagrams of the protein molecule states (native, intermediate and unfolded) are presented and discussed and the role of the intermediate state in protein folding is considered.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Cattle
  • Guanidine
  • Guanidines
  • Humans
  • Hydrogen-Ion Concentration
  • Lactalbumin*
  • Protein Conformation*
  • Spectrophotometry, Ultraviolet
  • Temperature

Substances

  • Guanidines
  • Lactalbumin
  • Guanidine