Cis-9,10-Methyleneoctadecanoic acid, one of a group of cyclopropane fatty acids commonly found in bacteria but not in eukaryotic cells, has been identified in the phosphatidylethanolamines of 27 isolates representing 5 genera of trypanosomatid flagellates (Crithidia, Leptomonas, Herpetomonas, Phytomonas, Leishmania). Its presence did not appear to be associated with endosymbiotic or other microbiol associates. It was absent from 12 isolates of the genera Blastocrithidia, Endotrypanum and Trypanosoma.