Abstract
To further define the canonical binding site for the P5-promoted Rep proteins of the adeno-associated virus, a modified random oligonucleotide selection procedure was performed, using purified recombinant Rep protein. These results may explain the effects of Rep on cellular gene expression.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Binding Sites
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Consensus Sequence
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Conserved Sequence
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DNA Primers
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DNA, Viral / chemistry
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DNA, Viral / metabolism
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DNA-Binding Proteins / metabolism*
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Dependovirus / metabolism*
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Molecular Sequence Data
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Oligonucleotide Probes / chemistry
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Oligonucleotide Probes / metabolism*
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Random Allocation
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Recombinant Proteins / metabolism
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Substrate Specificity
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Viral Proteins / metabolism*
Substances
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DNA Primers
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DNA, Viral
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DNA-Binding Proteins
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Oligonucleotide Probes
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Recombinant Proteins
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Viral Proteins
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rep proteins, Adeno-associated virus 2