Abstract
A mutation in the let-653 gene of Caenorhabditis elegans results in larval death. The lethal arrest is concurrent with the appearance of a vacuole anterior to the lower pharyngeal bulb. The position of the vacuole is consistent with a dysfunction of the secretory/excretory apparatus. Germline transformation rescue experiments were able to position the let-653 gene to two overlapping cosmid subclones. Sequence data generated from both cDNA and genomic DNA subclones indicated that let-653 encodes a mucin-like protein. Our characterization suggests that a mucin-like protein is essential for effective functioning of the secretory/excretory apparatus within C. elegans.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Caenorhabditis elegans / cytology
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Caenorhabditis elegans / genetics*
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Caenorhabditis elegans / physiology
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Caenorhabditis elegans Proteins*
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Cloning, Molecular
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Gene Expression / genetics
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Genes, Helminth*
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Helminth Proteins / chemistry
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Helminth Proteins / classification
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Helminth Proteins / genetics*
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Helminth Proteins / physiology
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Larva / cytology
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Larva / genetics
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Larva / growth & development
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Molecular Sequence Data
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Mucins / chemistry
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Mucins / classification
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Mucins / genetics*
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Mucins / physiology
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Mutation / genetics
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Phenotype
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RNA Splicing / genetics
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Restriction Mapping
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Sequence Analysis
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Transformation, Genetic / genetics
Substances
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Caenorhabditis elegans Proteins
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Helminth Proteins
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LET-653 protein, C elegans
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Mucins