Distributions of nitropyrenes and mutagenicity in airborne particulates collected with an Andersen sampler

Mutat Res. 1995 Oct;348(2):57-61. doi: 10.1016/0165-7992(95)00046-1.

Abstract

Distributions of 1,3-dinitropyrene (1,3-DNP), 1,6-DNP, 1,8-DNP, 1-nitropyrene (1-NP) and mutagenicity in airborne particulates collected in downtown Kanazawa, Japan with an Andersen high-volume air sampler were examined. Mutagenicities of benzene-ethanol extract from particulates were determined by the Ames test using S. typhimurium strains without S9 mix, while concentrations of DNPs and 1-NP were determined by high-performance liquid chromatography (HPLC) using chemiluminescence detection. In the finest particulate fraction (smaller than 1.1 microns), 68% and 75% of the total mutagenicities were observed in TA98 and YG1024 strains, respectively. In the same fraction, 65-82% of three DNPs as well as 84% of 1-NP were observed. Mutagenic contributions of 1,3-DNP, 1,6-DNP, 1,8-DNP and 1-NP in the extract were respectively 0.6, 1.2, 1.8 and 1.6% in the TA98 strain, and 2.5, 5, 9 and 2.1% in the YG1024 strain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Air Pollution / analysis*
  • Chromatography, High Pressure Liquid
  • Mutagenicity Tests
  • Mutagens / analysis*
  • Pyrenes / analysis*
  • Pyrenes / toxicity*
  • Salmonella typhimurium / drug effects

Substances

  • Mutagens
  • Pyrenes
  • 1,8-dinitropyrene
  • 1,6-dinitropyrene
  • 1,3-dinitropyrene
  • 1-nitropyrene