[Direct analysis of the frequency of disomy in human sperm using the PRINS technique]

I Quenesson; A Girardet; L Coignet; B Andréo; G Lefort; J P Charlieu; F Pellestor

[Direct analysis of the frequency of disomy in human sperm using the PRINS technique]

Ann Genet. 1995;38(2):85-9.

[Article in French]

Authors

I Quenesson¹, A Girardet, L Coignet, B Andréo, G Lefort, J P Charlieu, F Pellestor

Affiliation

¹ CNRS UPR 9008, BP 5051, Montpellier, France.

PMID: 7486830

Abstract

The PRINS method allows a rapid and specific detection of human chromosomes in situ. We have adapted the PRINS protocol to human sperm. Estimates of disomy have thus been performed for chromosomes 9, 12, 16 and 21 by using alpha-satellite DNA specific primers. The frequencies of disomy ranged from 0.27% to 0.31%. No significant difference was found. These data agree with the hypothesis of an equal distribution of non-disjunctions among chromosomes in male meiosis.

Publication types

English Abstract
Research Support, Non-U.S. Gov't

MeSH terms

Adult
Base Sequence
Biotin
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 16
Chromosomes, Human, Pair 21
Chromosomes, Human, Pair 9
DNA Primers*
DNA, Satellite / analysis*
Digoxigenin
Humans
In Situ Hybridization, Fluorescence / methods*
Male
Meiosis
Microscopy, Fluorescence
Molecular Sequence Data
Nondisjunction, Genetic*
Polymerase Chain Reaction / methods*
Spermatozoa / abnormalities*

Substances

DNA Primers
DNA, Satellite
Biotin
Digoxigenin