Abstract
Multiple serine/threonine kinases were detected in a bone marrow stromal cell line. One of these, the murine activin receptor like kinase-1 (ALK-1) homolog was cloned and sequenced. The expressed recombinant protein (62 kDa) was consistent in size with that predicted by the cDNA (58.6 kDa). On Western blots, a goat polyclonal antibody detected the native ALK-1 protein in bone marrow stromal cells, lung, brain, kidney and spleen. Two protein species of 60 kDa and 72-76 kDa were detected. Glycosylation events or alternative splicing may account for the larger protein specie.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Activin Receptors
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Amino Acid Sequence
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Animals
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Antibodies
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Base Sequence
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Blotting, Western
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Bone Marrow / metabolism
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Bone Marrow Cells
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Brain / metabolism
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Cell Line
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Cloning, Molecular
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DNA Primers
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Goats / immunology
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Humans
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Kidney / metabolism
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Lung / metabolism*
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Mice
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Molecular Sequence Data
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Organ Specificity
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Peptides / chemical synthesis
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Peptides / immunology
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Polymerase Chain Reaction
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Protein Biosynthesis
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Protein Serine-Threonine Kinases / biosynthesis*
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Protein Serine-Threonine Kinases / genetics
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Recombinant Proteins / biosynthesis
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Sequence Homology, Amino Acid
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Spleen / metabolism
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Transcription, Genetic
Substances
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Antibodies
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DNA Primers
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Peptides
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Recombinant Proteins
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Protein Serine-Threonine Kinases
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Activin Receptors