A novel Escherichia coli lipoprotein expression vector

T S Jones; V V Tryon

doi:10.1016/0378-1119(95)00496-s

A novel Escherichia coli lipoprotein expression vector

Gene. 1995 Nov 7;165(1):145-6. doi: 10.1016/0378-1119(95)00496-s.

Authors

T S Jones¹, V V Tryon

Affiliation

¹ Department of Microbiology, University of Texas Health Science Center at San Antonio 78284-7758, USA.

PMID: 7489907
DOI: 10.1016/0378-1119(95)00496-s

Abstract

A novel Escherichia coli (Ec) lipoprotein expression plasmid, pSJLP, was constructed. The plasmid contains a truncated alkaline phosphatase gene (phoA) located downstream from the Lac repressor gene lacIq and the IPTG inducible Ptac promoter. The phoA gene was truncated by deleting the native phoA signal sequence and fusing the truncated phoA gene to the lipoprotein signal sequence of the major Ec lipoprotein LPP. The recombinant LPP::PhoA fusion protein is produced and processed as a lipoprotein and can therefore be used as substrate for a novel signal peptidase II assay.

Publication types

Research Support, U.S. Gov't, P.H.S.

MeSH terms

Base Sequence
Escherichia coli / genetics*
Escherichia coli / metabolism
Genetic Vectors*
Lipoproteins / genetics*
Molecular Sequence Data
Plasmids / genetics*
Plasmids / isolation & purification

Substances

Lipoproteins