Determination of tissue inhibitor of metalloproteinases-2 (TIMP-2) in experimental animals using monoclonal antibodies against TIMP-2-specific oligopeptides

J Immunol Methods. 1995 Nov 16;187(1):33-9. doi: 10.1016/0022-1759(95)00164-6.

Abstract

We have developed a one-step sandwich enzyme immunoassay (EIA) using monoclonal antibodies against oligopeptides of the human tissue inhibitor of metalloproteinases-2 (TIMP-2) (Fujimoto et al. (1993) Clin. Chim. Acta 220, 31). The present studies further demonstrated that the antibodies cross-react with TIMP-2 species of experimental animals including mouse, rat, guinea pig and rabbit. The detection of the TIMP-2 species in our EIA system was verified using rat TIMP-2 and the EIA was subsequently used to measure the animal TIMP-2 in the sera. Using human TIMP-2 as a standard, TIMP-2 levels in the sera of mouse, rat, guinea pig and rabbit were approximately 80, 200, 270 and 25 ng/ml, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Cross Reactions
  • Guinea Pigs
  • Humans
  • Immunoblotting
  • Immunoenzyme Techniques / standards*
  • Mice
  • Protease Inhibitors / analysis*
  • Proteins / analysis*
  • Proteins / immunology
  • Rabbits
  • Rats
  • Tissue Inhibitor of Metalloproteinase-2

Substances

  • Antibodies, Monoclonal
  • Protease Inhibitors
  • Proteins
  • Tissue Inhibitor of Metalloproteinase-2