Abstract
The long-chain diacylglycerol 1,2-dimyristoylglycerol emulsified with taurodeoxycholate has been shown to potently stimulate glucose transport in isolated rat adipocytes (Stråfors, Nature 335, 554-556 (1988)). We now report that this 1,2-diacylglycerol in the presence of taurodeoxycholate, similarly to insulin, induced translocation of the insulin-regulated glucose transporter (GLUT-4) from a microsomal membrane compartment to the plasma membrane. H4IIE hepatoma cells expressed mRNA for GLUT-1, but not for GLUT-4. In these, otherwise insulin-responsive, cells diacylglycerol or insulin had only a marginal effect on glucose transport.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipocytes / drug effects
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Adipocytes / metabolism*
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Animals
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Base Sequence
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Biological Transport / drug effects
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Cell Membrane / metabolism
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Cells, Cultured
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Diglycerides / pharmacology*
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Excipients
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Glucose / metabolism
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Glucose Transporter Type 1
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Glucose Transporter Type 4
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Insulin / pharmacology*
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Liver Neoplasms, Experimental / metabolism
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Microsomes / metabolism
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Molecular Sequence Data
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Monosaccharide Transport Proteins / genetics
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Monosaccharide Transport Proteins / metabolism*
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Muscle Proteins*
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RNA, Messenger / analysis
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Rats
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Rats, Sprague-Dawley
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Taurodeoxycholic Acid
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Tumor Cells, Cultured
Substances
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1,2-diacylglycerol
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Diglycerides
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Excipients
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Glucose Transporter Type 1
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Glucose Transporter Type 4
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Insulin
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Monosaccharide Transport Proteins
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Muscle Proteins
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RNA, Messenger
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Slc2a1 protein, rat
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Slc2a4 protein, rat
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Taurodeoxycholic Acid
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Glucose