Abstract
Human helper T-cell (Th) responses to influenza A virus were studied by analyzing T-cell receptor V beta gene diversity in hemagglutinin-specific Th lymphocytes. The T-lymphocyte population from peripheral blood became quickly oligoclonal when stimulated in vitro with the HA306-329 peptide, and T-cell receptor V beta 3 genes were mainly expanded. Moreover, specific junctional region oligonucleotide probes corresponding to hemagglutinin-specific clones were used to estimate temporal diversity during antigenic stimulations.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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CD4-Positive T-Lymphocytes / immunology*
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Cell Line
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Clone Cells
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DNA Primers
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HLA-DR Antigens / immunology
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Hemagglutinin Glycoproteins, Influenza Virus
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Hemagglutinins, Viral / chemistry
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Hemagglutinins, Viral / immunology*
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Humans
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Influenza A virus / immunology*
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Molecular Sequence Data
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Polymerase Chain Reaction
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Receptor-CD3 Complex, Antigen, T-Cell / immunology
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Receptors, Antigen, T-Cell, alpha-beta / genetics
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Receptors, Antigen, T-Cell, alpha-beta / immunology
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T-Lymphocytes, Helper-Inducer / immunology*
Substances
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DNA Primers
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HLA-DR Antigens
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Hemagglutinin Glycoproteins, Influenza Virus
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Hemagglutinins, Viral
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Receptor-CD3 Complex, Antigen, T-Cell
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Receptors, Antigen, T-Cell, alpha-beta