Abstract
The configuration of latent varicella-zoster virus (VZV) DNA was analyzed by PCR. Template DNA for both internal and terminal VZV primers was present in a 1:1 ratio in ganglionic DNA, compared with a 15:1 ratio in DNA extracted from VZV virions, indicating that the VZV genomic termini are adjacent in latently infected human ganglia.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adult
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Aged
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Animals
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Base Sequence
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Cell Line
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Cloning, Molecular
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DNA Primers
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DNA, Viral / chemistry*
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DNA, Viral / isolation & purification
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Female
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Herpesvirus 3, Human / genetics*
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Herpesvirus 3, Human / isolation & purification
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Herpesvirus 3, Human / physiology
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Humans
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Male
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Middle Aged
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Molecular Sequence Data
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Nucleic Acid Conformation
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Polymerase Chain Reaction
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Restriction Mapping
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Trigeminal Ganglion / virology*
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Virus Latency