Occurrence, in Crohn's disease, of antibodies directed against a species-specific recombinant polypeptide of Mycobacterium paratuberculosis

P Vannuffel; C Dieterich; B Naerhuyzen; P Gilot; M Coene; R Fiasse; C Cocito

doi:10.1128/cdli.1.2.241-243.1994

Occurrence, in Crohn's disease, of antibodies directed against a species-specific recombinant polypeptide of Mycobacterium paratuberculosis

Clin Diagn Lab Immunol. 1994 Mar;1(2):241-3. doi: 10.1128/cdli.1.2.241-243.1994.

Authors

P Vannuffel¹, C Dieterich, B Naerhuyzen, P Gilot, M Coene, R Fiasse, C Cocito

Affiliation

¹ Microbiology and Genetics Unit, University of Louvain, Brussels, Belgium.

Abstract

Sera from patients with Crohn's disease and control were analyzed by an enzyme-linked immunosorbent assay based on the Mycobacterium paratuberculosis-specific recombinant polypeptide a362. Anti-a362 immunoglobulin G (IgG) (P < 0.05) and IgA (P < 0.001) titers were higher in patients with Crohn's disease than in controls. A monomodal Gaussian distribution of anti-a362 IgA levels were found for controls, and a bimodal distribution was found for patients with Crohn's disease. An M. paratuberculosis etiology is suggested for the 36% of patients with Crohn's disease who had an anti-a362 IgA level higher than that of controls.

MeSH terms

Antibodies, Bacterial / chemistry*
Antigens, Bacterial / immunology*
Crohn Disease / immunology*
Crohn Disease / microbiology
Humans
Mycobacterium avium subsp. paratuberculosis / immunology*
Peptides / immunology*
Recombinant Proteins / immunology
Species Specificity

Substances

Antibodies, Bacterial
Antigens, Bacterial
Peptides
Recombinant Proteins