Abstract
In our search for genes up- or down-regulated genes in the mdx mouse model for Duchenne muscular dystrophy, we isolated a down-regulated mitochondrial DNA clone. In addition to this clone, all protein-coding mitochondrial genes tested had tissue-specific and age independent down-regulated expression. This implied mechanisms at the RNA level since no change in the mitochondrial DNA contents were detected. Cytochrome c oxidase activity showed the same range of down-regulated expression. These data provide a molecular basis for energetic metabolism modifications in mdx mice.
MeSH terms
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Aging / metabolism*
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Animals
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Base Sequence
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Blotting, Northern
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DNA Primers
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Electron Transport Complex IV / biosynthesis
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Electron Transport Complex IV / metabolism*
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Gene Expression Regulation
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Mice
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Mice, Inbred C57BL
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Mice, Inbred mdx
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Mitochondria, Muscle / metabolism*
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Molecular Sequence Data
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Muscle Development
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Muscle, Skeletal / growth & development
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Muscle, Skeletal / metabolism
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Muscular Dystrophy, Animal / genetics*
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Muscular Dystrophy, Animal / metabolism*
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Organ Specificity
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Polymerase Chain Reaction
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RNA / biosynthesis*
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RNA, Messenger / biosynthesis*
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RNA, Mitochondrial
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Reference Values
Substances
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DNA Primers
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RNA, Messenger
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RNA, Mitochondrial
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RNA
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Electron Transport Complex IV