Abstract
The tetratricopeptide repeat (TPR) is a 34-amino-acid degenerate sequence motif that is found in a large variety of proteins, both prokaryotic and eukaryotic. TPRs are usually found in tandem arrays of up to 16 copies. In this paper we identify a direct interaction between the TPRs of Ssn6, a general transcriptional repressor, and alpha 2, a cell-type regulator in Saccharomyces cerevisiae. Five of the Ssn6 TPRs were tested individually, and all were found to interact specifically with alpha 2. These results suggest a model for TPR-protein interactions and for the role that a tandem array of TPRs may have in mediating transcriptional repression.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Binding Sites
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Chromatography, Affinity
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Conserved Sequence
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Fungal Proteins / chemistry
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Glutathione Transferase
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Homeodomain Proteins*
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Models, Chemical
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Nuclear Proteins*
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism
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Repressor Proteins*
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins*
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Substrate Specificity
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tau Proteins / metabolism
Substances
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CYC8 protein, S cerevisiae
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DNA-Binding Proteins
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Fungal Proteins
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Homeodomain Proteins
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MATA2 protein, S cerevisiae
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Nuclear Proteins
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Recombinant Proteins
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Repressor Proteins
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Saccharomyces cerevisiae Proteins
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tau Proteins
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Glutathione Transferase