Abstract
We have isolated a cDNA for a novel human amiloride-sensitive Na+ channel isoform (called delta) which is expressed mainly in brain, pancreas, testis, and ovary. When expressed in Xenopus oocytes, it generates an amiloride-sensitive Na+ channel with biophysical and pharmacological properties distinct from those of the epithelial Na+ channel, a multimeric assembly of alpha, beta, and gamma subunits. The Na+ current produced by the new delta isoform is increased by two orders of magnitude after coexpression of the beta and gamma subunit of the epithelial Na+ channel showing that delta can associate with other subunits and is part of a novel multisubunit ion channel.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amiloride / pharmacology*
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular / methods
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DNA Primers
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Electrophysiology
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Female
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Gene Library
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Humans
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Kidney / metabolism
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Macromolecular Substances
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Male
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Membrane Potentials / drug effects
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Molecular Sequence Data
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Oocytes / drug effects
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Oocytes / physiology
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Organ Specificity
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Polymerase Chain Reaction
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / chemistry
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Recombinant Proteins / drug effects
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Sequence Homology, Amino Acid
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Sodium Channels / biosynthesis*
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Sodium Channels / chemistry
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Sodium Channels / drug effects
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Xenopus
Substances
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DNA Primers
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Macromolecular Substances
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RNA, Messenger
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Recombinant Proteins
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Sodium Channels
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Amiloride