Kinetics of the bovine beta-trypsin (trypsin) reaction with the active site titrant N alpha-(N,N-dimethylcarbamoyl)- alpha-aza-ornithine p-nitrophenyl ester (Dmc-azaOrn-ONp) was obtained at pH 6.2 and 21.0 degrees C. The results are consistent with the minimum three-step catalytic mechanism of serine proteinases involving a stable acyl.enzyme adduct. Dmc-azaOrn-ONp binds stoichiometrically to trypsin and allows the reliable determination of the active enzyme concentration between 1.0 x 10(-6) M and 3.0 x 10(-4) M. The three-dimensional structure of the trypsin.Dmc-azaOrn acyl.enzyme adduct has been solved by X-ray crystallography at 1.8 A resolution (R = 0.153). The Dmc-azaOrn moiety of the active site titrant is accommodated in the serine proteinase active center, occupying the S1 specificity subsite, and is covalently linked to the OG atom of the Ser195 catalytic residue.