Functional and phenotypical analysis of subsets of rat CD4+ T cells

Microbiol Immunol. 1993;37(8):623-32. doi: 10.1111/j.1348-0421.1993.tb01685.x.

Abstract

Rat CD4+ T cells were divided into two distinct subsets by a monoclonal antibody RTH-1 recognizing a unique epitope on rat CD45R. Cellular distribution of OX-22- and RTH-1-defined antigens was the same. However, OX-22 and RTH-1 recognized different epitopes that exist on rat CD45R. The expression of IL-4 gene was detected only in RTH-1low CD4+ T cell subset upon various stimulations. In contrast, the expression of IL-2 and IFN-gamma gene varied depending upon the nature of stimuli. The increased cell surface expression of CD44 was detected in RTH-1high CD4+ T cell subset. Conversely the increased expression of CD2 was detected in RTH-1low CD4+ T cell subset. The expression of CD3 and LFA-1 was not significantly different between RTH-1high and RTH-1low subsets.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Base Sequence
  • CD4-Positive T-Lymphocytes* / immunology
  • CD4-Positive T-Lymphocytes* / physiology
  • Gene Expression
  • Hyaluronan Receptors
  • Immunophenotyping
  • Interferon-gamma / biosynthesis
  • Interleukin-2 / biosynthesis
  • Interleukin-4 / biosynthesis
  • Leukocyte Common Antigens / analysis
  • Mice
  • Mice, Inbred BALB C / immunology
  • Molecular Sequence Data
  • Rats / immunology*
  • Rats, Inbred F344 / immunology
  • Rats, Inbred Lew / immunology
  • Receptors, Lymphocyte Homing / analysis

Substances

  • Antibodies, Monoclonal
  • Hyaluronan Receptors
  • Interleukin-2
  • Receptors, Lymphocyte Homing
  • Interleukin-4
  • Interferon-gamma
  • Leukocyte Common Antigens