Abstract
Two kidney water channels have been identified: CHIP28 in proximal tubule and thin descending limb, and WCH-CD in collecting duct apical membrane. An homologous cDNA (WCH3) was obtained from rat kidney and found to encode a 276 amino acid, 29 kDa protein with 39% amino acid identity to rat CHIP28, 50% to WCH-CD and 49% to MIP26. The WCH3 transcript of 2.5 kb was expressed exclusively in kidney and was upregulated in dehydrated rats. Cell-free translation produced an approximately 28 kDa protein. Analysis of the predicted amino acid sequence indicated a hydrophobic protein with 4-6 membrane-spanning domains, with one N-linked glycosylation site, two conserved NPA boxes common to MIP26 family proteins, and conserved residue C189 common to water channels. WCH3 is a new member of the MIP26 family of channel-forming proteins in mammalian kidney.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Aquaporin 1
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Aquaporin 2
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Aquaporin 6
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Aquaporins*
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Base Sequence
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Cloning, Molecular
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Conserved Sequence
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DNA Primers
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DNA, Complementary / metabolism*
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Gene Library
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Ion Channels / biosynthesis*
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Kidney / metabolism*
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Kidney Cortex / metabolism
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Kidney Medulla / metabolism
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Kidney Tubules, Proximal / metabolism
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Membrane Proteins / biosynthesis*
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Membrane Proteins / chemistry
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Molecular Sequence Data
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Oligonucleotides, Antisense
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Polymerase Chain Reaction
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Protein Biosynthesis
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Protein Conformation
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RNA, Messenger / metabolism
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Rats
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Sequence Homology, Amino Acid
Substances
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Aqp1 protein, rat
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Aqp2 protein, rat
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Aquaporin 2
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Aquaporin 6
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Aquaporins
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DNA Primers
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DNA, Complementary
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Ion Channels
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Membrane Proteins
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Oligonucleotides, Antisense
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RNA, Messenger
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Aquaporin 1
Associated data
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GENBANK/D17642
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GENBANK/D17796
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GENBANK/D17797
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GENBANK/D17798
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GENBANK/D17799
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GENBANK/D17800
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GENBANK/D17801
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GENBANK/D17802
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GENBANK/L28113
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GENBANK/U04828