Abstract
Fusion of BERH-2 rat hepatocellular carcinoma cells with activated B cells produced hybrid cells that lost their tumorigenicity and became immunogenic. Syngeneic rats injected with BERH-2-B hybrid cells became resistant to challenge with parental BERH-2 cells, and rats with established BERH-2 hepatomas were cured by subsequent injection of BERH-2-B cells. Both CD4+ and CD8+ cells were essential for the induction of protective immunity; however, only CD8+ cells were required for the eradication of BERH-2 tumors. The generation of hybrid tumor cells that elicit antitumor immune responses may be a useful strategy for cancer immunotherapy.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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B-Lymphocytes / immunology*
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B7-1 Antigen / analysis
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CD4-Positive T-Lymphocytes / immunology
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Cell Fusion
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Female
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Histocompatibility Antigens Class II / analysis
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Hybrid Cells / immunology*
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Immunotherapy, Active
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Liver Neoplasms, Experimental / immunology*
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Liver Neoplasms, Experimental / prevention & control
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Liver Neoplasms, Experimental / therapy
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Lymphocyte Activation
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Neoplasm Transplantation
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Rats
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Rats, Wistar
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T-Lymphocyte Subsets / immunology
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Tumor Cells, Cultured
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Vaccination
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Vaccines / immunology*
Substances
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B7-1 Antigen
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Histocompatibility Antigens Class II
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Vaccines