Abstract
Monoclonal L3 and L4 antibodies have been shown to recognize carbohydrate epitopes on several neural cell adhesion molecules; these epitopes can be released by treatment with endoglycosidase H. In the present study, we have identified the oligosaccharides released by endoglycosidase H from the cell adhesion molecules AMOG and L1 by fast-atom bombardment mass spectrometry as being solely of the oligomannosidic type. Using neoglycolipids of oligomannosidic glycans, we also report that both antibodies show the highest reactivity with the alpha-manno-pentaose Man alpha 1-3-[Man alpha 1-6(Man alpha 1-3)Man alpha 1-6]-Man, but decreasing reactivity with the alpha-manno-hexaose, heptaose, octaose and nonaose glycans. Thus, to our knowledge, we describe here for the first time monoclonal antibodies recognizing N-glycosidically linked oligomannosidic glycans.
MeSH terms
-
Adenosine Triphosphatases
-
Animals
-
Antibodies, Monoclonal / biosynthesis
-
Antibodies, Monoclonal / immunology*
-
Binding Sites, Antibody
-
Carbohydrate Sequence
-
Cation Transport Proteins
-
Cell Adhesion Molecules, Neuronal / chemistry
-
Cells, Cultured
-
Cerebellum / cytology
-
Cerebellum / immunology*
-
Enzyme-Linked Immunosorbent Assay
-
Epitopes / analysis
-
Epitopes / immunology
-
Extracellular Matrix Proteins / chemistry
-
Fucose / chemistry
-
Membrane Glycoproteins / chemistry
-
Membrane Glycoproteins / immunology*
-
Mice
-
Molecular Sequence Data
-
Neurons / cytology
-
Neurons / drug effects
-
Neurons / metabolism
-
Oligosaccharides / immunology
-
Polysaccharides / chemistry
-
Polysaccharides / immunology*
-
Spectrometry, Mass, Fast Atom Bombardment
-
Swainsonine / pharmacology
Substances
-
Antibodies, Monoclonal
-
Atp1b2 protein, mouse
-
Cation Transport Proteins
-
Cell Adhesion Molecules, Neuronal
-
Epitopes
-
Extracellular Matrix Proteins
-
Membrane Glycoproteins
-
Oligosaccharides
-
Polysaccharides
-
oligomannoside
-
Fucose
-
Adenosine Triphosphatases
-
Swainsonine