Z-RNA. The synthesis of 2'-O-[13C]methyl- and 5-methyl-analogs of ribo-CGCGCG

E Biała; J Milecki; A Kowalewski; M Popenda; W Z Antkowiak; R W Adamiak

Z-RNA. The synthesis of 2'-O-[13C]methyl- and 5-methyl-analogs of ribo-CGCGCG

Acta Biochim Pol. 1993;40(4):521-30.

Authors

E Biała¹, J Milecki, A Kowalewski, M Popenda, W Z Antkowiak, R W Adamiak

Affiliation

¹ Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.

PMID: 7511312

Abstract

Chemical synthesis of 2'-O-[13C]methyl-rCGCGCG and 5-methyl-rCGCGCG using support-aided phosphoramidite method is presented. 2'-O-Methyl guanosine derivative was separated from its 3'-O-methyl counterpart using transient 5',3'-O-silylation with 1,3-dichloro-1,1,3,3-tetraisopropyldisiloxane (Markiewicz reagent). The hexamers were obtained in a purity suitable for NMR studies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Carbon Isotopes
Methods
Methylation
Nucleic Acid Conformation
Oligoribonucleotides / chemical synthesis*
Oligoribonucleotides / chemistry
RNA / chemical synthesis*
RNA / chemistry

Substances

Carbon Isotopes
Oligoribonucleotides
RNA