Abstract
A method for detecting hyaluronan (HA)-binding proteins in transblot assays using biotinylated HA (BHA) is described. Some of the binding characteristics of a novel HA receptor termed RHAMM (Receptor for HA-Mediated Motility) are characterized using this assay. The method is also used to detect other HA-binding proteins in tissue homogenates. This method is semiquantitative, rapid, reproducible, sensitive and therefore of potential use in identifying the levels of HA-binding proteins in different cells and tissues.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Biotin / metabolism
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Carrier Proteins / analysis*
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Electrophoresis, Polyacrylamide Gel / methods
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Hyaluronan Receptors
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Hyaluronic Acid / analysis
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Methods
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Molecular Probes / analysis
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Rats
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Rats, Sprague-Dawley
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Receptors, Cell Surface / analysis*
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Receptors, Lymphocyte Homing / analysis*
Substances
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Carrier Proteins
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Hyaluronan Receptors
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Molecular Probes
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Receptors, Cell Surface
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Receptors, Lymphocyte Homing
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Biotin
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Hyaluronic Acid