Abstract
Molecular cloning of the dog homologue of the human CD44 was achieved using RT/PCR. A 1055 bp cDNA has a deduced amino acid sequence of 351 residues, 338 of them correspond to the mature protein. Nine conserved cysteine residues were found. The extracellular region contains a single link superfamily domain on the N-terminal part and potential post-translational modification sites as: N- and O-linked glycosylation sites and chondroitin sulfate attachment sites. Three mRNAs of 2.2, 3.8 and 4.4 kb were identified on Northern blot analysis and Western blot hybridization revealed a 85-90 kDa protein expressed in lymph node tissue.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Blotting, Western
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Chondroitin Sulfates / metabolism
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Cloning, Molecular
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Dogs
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Glycosylation
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Humans
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Hyaluronan Receptors
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Molecular Sequence Data
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Protein Processing, Post-Translational
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Receptors, Cell Surface / genetics*
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Receptors, Cell Surface / metabolism
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Receptors, Lymphocyte Homing / genetics*
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Receptors, Lymphocyte Homing / metabolism
Substances
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Carrier Proteins
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Hyaluronan Receptors
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Receptors, Cell Surface
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Receptors, Lymphocyte Homing
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Chondroitin Sulfates