Abstract
Two molecular mechanisms of T cell-mediated cytotoxicity, one perforin-based, the other Fas-based, have been demonstrated. To determine the extent of their contribution to T cell-mediated cytotoxicity, a range of effector cells from normal control or perforin-deficient mice were tested against a panel of target cells with various levels of Fas expression. All cytotoxicity observed was due to either of these mechanisms, and no third mechanism was detected. Thus, the perforin- and Fas-based mechanisms may account for all T cell-mediated cytotoxicity in short-term in vitro assays.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Surface / immunology*
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Cells, Cultured
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Concanavalin A / pharmacology
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Cytotoxicity, Immunologic*
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Ionomycin / pharmacology
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Leukemia L1210
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Lymphocyte Culture Test, Mixed
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Lymphocytic choriomeningitis virus / immunology
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Membrane Glycoproteins / immunology*
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Mice
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Mice, Inbred BALB C
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Mice, Inbred C3H
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Mice, Inbred C57BL
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Mice, Mutant Strains
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Molecular Sequence Data
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Perforin
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Pore Forming Cytotoxic Proteins
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T-Lymphocytes, Cytotoxic / immunology*
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Tetradecanoylphorbol Acetate / pharmacology
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Tumor Cells, Cultured
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fas Receptor
Substances
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Antigens, Surface
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Membrane Glycoproteins
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Pore Forming Cytotoxic Proteins
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fas Receptor
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Concanavalin A
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Perforin
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Ionomycin
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Tetradecanoylphorbol Acetate