Abstract
Uptake of the nitric oxide synthase inhibitors NG-methyl-L-arginine (L-NMA) and NG-nitro-L-arginine (L-NNA) by macrophages is mediated by two different mechanisms. Activation of the cells with cytokines resulted in an up-regulation of L-NMA uptake but did not affect L-NNA transport. Characterization of the transport sites revealed that uptake of L-NMA is mediated by a cationic amino acid transporter (system y+) whereas a neutral amino acid transporter (system L) accounts for the uptake of L-NNA.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Oxidoreductases / antagonists & inhibitors*
-
Amino Acid Transport Systems
-
Animals
-
Arginine / analogs & derivatives*
-
Arginine / metabolism
-
Biological Transport
-
Carrier Proteins / antagonists & inhibitors
-
Carrier Proteins / metabolism
-
Cell Line
-
Interferon-gamma / pharmacology
-
Leucine / metabolism
-
Lipopolysaccharides / pharmacology
-
Macrophage Activation
-
Macrophages / metabolism*
-
Mice
-
Nitric Oxide Synthase
-
Nitroarginine
-
Recombinant Proteins / pharmacology
-
Sodium / pharmacology
-
omega-N-Methylarginine
Substances
-
Amino Acid Transport Systems
-
Carrier Proteins
-
Lipopolysaccharides
-
Recombinant Proteins
-
Nitroarginine
-
omega-N-Methylarginine
-
Interferon-gamma
-
Arginine
-
Sodium
-
Nitric Oxide Synthase
-
Amino Acid Oxidoreductases
-
Leucine