Benzoporphyrin derivative: a potent photosensitizer for photodynamic destruction of rabbit endometrium

Obstet Gynecol. 1994 Sep;84(3):409-14.

Abstract

Objectives: To determine the optimal pharmacokinetic characteristics for photodynamic endometrial destruction using topically applied benzoporphyrin derivative and to evaluate long-term morphologic effects in a rabbit model.

Methods: Benzoporphyrin derivative pharmacokinetics were measured by analyzing frozen tissue sections 1.5-12 hours following topical administration. Photodynamic therapy was induced intraluminally 1.5 hours after drug application, and tissue morphology was evaluated by light and scanning electron microscopy 1 and 4 weeks after treatment.

Results: The highest glandular and stromal fluorescence was observed 1.5 hours after topical administration. Relative fluorescence of the endometrial glands significantly exceeded that of stroma and myometrium mainly at 1.5 and 3 hours (P < .0001). Histology revealed persistent epithelial destruction with minimal regeneration. Stroma resembled scar tissue in the majority of sections. The bordering myometrium was loosened and invaded by connective tissue.

Conclusion: Topically applied benzoporphyrin derivative can be used for highly effective, long-lasting photodynamic destruction of rabbit endometrium. However, optical dosimetry can vary, particularly in the rabbit model, and this appears to affect long-term reepithelialization.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Administration, Topical
  • Animals
  • Dextrans
  • Endometrium / drug effects*
  • Endometrium / ultrastructure
  • Female
  • Microscopy, Electron, Scanning
  • Photochemotherapy*
  • Porphyrins / pharmacokinetics*
  • Porphyrins / pharmacology
  • Rabbits
  • Radiation-Sensitizing Agents / pharmacokinetics*
  • Radiation-Sensitizing Agents / pharmacology

Substances

  • Dextrans
  • Porphyrins
  • Radiation-Sensitizing Agents
  • benzoporphyrin D