Synthesis of di- to penta-saccharides related to the O-specific polysaccharide of Shigella dysenteriae type 1, and their nuclear magnetic resonance study

Bioorg Med Chem. 1993 Oct;1(4):237-57. doi: 10.1016/s0968-0896(00)82129-x.

Abstract

The syntheses of oligosaccharide fragments of the O-specific polysaccharide of the lipopolysaccharide of Shigella dysenteriae type 1 are described, including disaccharides methyl O-alpha-D-mannopyranosyl-(1-->2)-alpha-D-galactopyranoside (1), and methyl O-(2-deoxy-2-propionamido-alpha-D-glucopyranosyl)-(1-->3)-alpha-L- rhamnopyranoside (2), trisaccharide methyl O-alpha-D-galactopyranosyl-(1-->3)-O-(2-acetamido-2-deoxy-alpha-D- glucopyranosyl)-(1-->3)-alpha-L-rhamnopyranoside (3), tetrasaccharide methyl O-alpha-L-rhamnopyranosyl-(1-->2)-O-alpha-D-galactopyranosyl-(1-->3)- O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-(1-->3)-alpha-L-rhamno -pyranoside (4), and pentasaccharide methyl O-alpha-L-rhamnopyranosyl-(1-->3)-O-alpha-L- rhamnopyranosyl-(1-->2)-O-alpha-D-galactopyranosyl- (1-->3)-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-(1-->3)-alpha-L- rhamnopyranoside (5). The following monosaccharide building blocks were used as starting compounds: methyl 6-O-tert-butyldiphenylsilyl-3,4-O-isopropylidene-alpha-D-galact opy ranoside (8), methyl 3,4,6-tri-O-benzyl-2-O-(4-methoxybenzyl)-1-thio-beta-D- galactopyranoside (11), methyl 3,4,6-tri-O-acetyl-2-azido-2-deoxy-1-thio-alpha- D-glucopyranoside (16), methyl 2-azido-4,6-O-benzylidene-2-deoxy-1-thio-alpha-D- glucopyranoside (18), methyl 2,4-di-O-benzyl-alpha-L-rhamnopyranoside (21), methyl 2,3,4-tri-O-benzoyl-1-thio-alpha-L-rhamnopyranoside (22), 2,3,4-tri-O-benzoyl-alpha-L-rhamnopyranosyl bromide (23), and methyl 4-O-benzyl-alpha-L-rhamnopyranoside (24). Nuclear magnetic resonance data indicate that oligosaccharides 4 and 5 partially mimic the conformation of the O-specific polysaccharide of S. dys. type 1.

MeSH terms

  • Antigens, Bacterial / chemistry*
  • Bacterial Vaccines / chemical synthesis
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Dysentery, Bacillary / prevention & control
  • Humans
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Molecular Structure
  • O Antigens
  • Oligosaccharides / chemical synthesis*
  • Oligosaccharides / chemistry
  • Oligosaccharides / immunology
  • Polysaccharides, Bacterial / chemistry*
  • Polysaccharides, Bacterial / immunology
  • Shigella dysenteriae / chemistry*
  • Shigella dysenteriae / classification
  • Shigella dysenteriae / immunology

Substances

  • Antigens, Bacterial
  • Bacterial Vaccines
  • O Antigens
  • Oligosaccharides
  • Polysaccharides, Bacterial